Gen-probe, Inc.

Biomerieux Sa

Other

Biocontrol Systems, Inc.

Blomorloux S.a.

Markus T Jucker

Steven T Brentano

Philippe L Cleuziat

Francisco D Delgado

Marc Rodrigue

Philippe Cleuziat

Philip Thomas Feldsine

Andrew Lienau

The present invention relates generally to methods for detecting and identifying microorganisms and, more particularly, to methods for detecting microorganisms in a sample by incubating the sample at two temperatures to facilitate increased detection of the organism.

Method for detecting pathogens

Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genusare disclosed. Kits including such oligonucleotides are disclosed. Methods of detectingspecies using the oligonucleotides in in vitro nucleic acid amplification are disclosed.

Nucleic acid amplification and detection of mycobacterium species

A method of detecting rpoB sequences ofpresent in a biological sample that includes steps of amplifying therpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences ofin a sample are disclosed.

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A method of detecting rpoB sequences ofpresent in a biological sample that includes steps of amplifying therpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences using multiple probes that provide sequence information by their specific hybridization to portions of the amplified nucleic acid. Compositions for amplifying and detecting in vitro the rpoB sequences ofin a sample are disclosed.

Detection of rpoB sequences of Mycobacterium tuberculosis

Methods of detecting Mycobacterium species using oligonucleotides to amplify in vitro 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species in the genus Mycobacterium are disclosed. Amplification oligonucleotides for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed. Kits containing oligonucleotides useful for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed.

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Poway, CA, United States of America

Markus T Jucker