Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Mascoma Corporation

Universiteit Stellenbosch

Mark Mellon

John E McBride

Elena E Brevnova

Erin Wiswall

Anne K Warner

Chhayal V Gandhi

Vineet Rajgarhia

Allan C Froehlich

Riaan Den Haan

Emily Stonehouse

Haowen Xu

Kristen M Deleault

John S Bardsley

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Charles F Rice

Ryan Skinner

Shital A Tripathi

Frank Agbogbo

Kevin S Wenger

Indraneel Shikhare

Yin-Ying Chiu

Jennifer Gosselin

Vineet B Rajgarhia

Alan Belcher

Abigail Foster

Heidi H Hau

Merja Elisa Penttila

Matti Siika-Aho

Marja Ilmen

Alan Benjamin Gilbert

Sanni Voutilainen

Anu Koivula

David Anthony Hogsett

Jaana Uusitalo

Deidre Willes

Emile Van Zyl

Shaunita Hellouise Rose

Danie Lagrange

Elena Brevnova

Naomi Thorngren

Heidi Lau

Jim Flatt

Jeff Villari

Merja Penttila

Abigail S Foster

Chhayal Ghandi

John Mcbride

Jeffrey D Keating

Emily A Stonehouse

Heidi Hanson Hau

Dan Murphy

Alan Froehlich

Shaunita Rose

John E Mcbride

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of termite cellulases in yeast

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Yeast cells expressing an exogenous cellulosome and methods of using the same

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Heterologous expression of termite cellulases yeast

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Grantham, NH, United States of America

Mark Mellon