Biomerieux, Inc.

Biomerieux Sa

Marie Cellier

Sylvain Orenga

John Perry

David Mosticone

Antoine Vimont

Marie-Pierre Bourguignon

Diane Halimi

John Mills

Denis Robichon

Rosaleen Joy Anderson

Keng Tiong Ng

Linda Varadi

Veronique Sauvonnet

Alexandre F Bedernjak

Véronique Sauvonnet

John D Perry

Linda VÁRADI

A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising:

Microorganism detection method comprising at least one alkyl(thio)glycoside

Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample likely to contain them, such as a food sample.

Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample

Use of at least one chromogenic and/or fluorogenic carboxylesterase and/or triacylglycerol-lipase substrate, to detect bacteria of thegroup in a sample capable of containing them.

[object Object]

Micro-organism detection medium comprising at least one alkyl(thio)glycoside

An antimicrobial compound, as well as the salts, derivatives and analogs thereof, said compound being represented by the general formula (I): wherein Rrepresents a peptide part P1 or a peptide part P2.

Antimicrobial compounds

The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of thegroup comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.

The present invention relates to a method for identifying bacteria of thegroup, comprising the following steps: (a) providing a sample that may contain bacteria of thegroup, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of thegroup by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of thegroup is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to thegroup, that may be present in the sample.

A reaction medium for the culture and/or detection and/or identification of bacteria of thegenus includes at least one siliceous compound, where the siliceous compound is a nonpolar silica. A method for detecting and/or identifying bacteria of thegenus, includes bringing a sample that may contain bacteria of thegenus into contact with a reaction medium comprising at least one siliceous compound, incubating, and detecting the presence of bacteria of thegenus.

Growing community of inventors

Montalieu-Vercieu, France

Marie Cellier