Consejo Superior De Investigaciones Cientificas

Institut Pasteur

Margarita Salas Falgueras

Patrick Forterre

Gemma Serrano De Las Heras

Jose M Lazaro Bolos

Mart Krupovic

Alicia Bravo Garcia

Mario Mencia Caballero

Luis Blanco Davila

Miguel De Vega Jose

Modesto Redrejo Rodriguez

Jose Mª Lazaro Bolos

Miguel De Vega JosÉ

José Mª Lazaro Bolos

The present invention provides an isolated peptide of SEQ ID NO: 1 needed for primase active as well as new replicative DNA polymerase enzymes, preferably that of SEQ ID NO: 2, comprising said peptide. Thus, these DNA polymerases are endowed with priming activity and do not require externally provided primers for initiating and performing DNA amplification. These polymerases are able to carry out a faithful and processive de novo DNA synthesis of DNA templates in the absence of pre-synthetized primers. Therefore, these enzymes of the invention act both as primases and DNA polymerases. Furthermore, they show translesion synthesis capacity, so that they may be useful not only for whole-genome amplification but also for the amplification of damaged DNAs. The invention further refers to methods for amplifying templates DNAs using these enzymes.

Primer-independent DNA polymerases and their use for DNA synthesis

A DNA polymerase chimera comprising an amino-terminal (N-terminal) region encoding a &#x3a6;29 type DNA polymerase and a carboxyl-terminal (C-terminal) region comprising at least one HhH domain which are bound by a connecting amino acid sequence is disclosed along with and the use thereof for replicating, amplifying or sequencing a template DNA. Also disclosed is a method for replicating, amplifying or sequencing a deoxyribonucleic acid with the DNA polymerase chimera and kits for carrying out the methods.

Phage phi29 DNA polymerase chimera

The present invention relates to a protein which has the capacity to bind to and inhibit the viral uracil DNA glycosylase (UDG) enzyme and its use as a therapeutic agent; in particular, as an antiviral agent.

Inhibitor of the uracil-DNA glycosylase enzyme and uses thereof

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Madrid, Spain

Margarita Salas Falgueras