Msd Wellcome Trust Hilleman Laboratories Pvt. Ltd.

Manoj Kumar Chhikara

Davinder Gill

Neeraj Joshi

Sarmad Hanif

Juned Dalal

Rakesh Rana

Deepti Singh

Kishore Harale

Sandeep Sharma

The present invention relates to synthesis of novel higher oligomers and process of preparing the same. In particular the present invention relates to the chemical synthesis of oligomers ofserogroup X ('hereinafter Men-X), more particularly tetramer. The present invention provides Men-X capsular oligomers obtained from synthetic pathway using purified saccharides of specific chain length and provides said novel oligomers as candidates for the development of conjugate vaccine against bacterial meningitis caused due to Men-X infections.

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The present invention relates to an improved process of conjugation to obtain synthetic oligosaccharide-protein (OS-PR) conjugates. The process of synthetic OS-PR conjugation is a rapid process providing oligosaccharide-protein conjugates which are highly immunogenic and elicit specific and homogenous immune responses. The synthetic oligosaccharide comprising of four to eight repeating units of respective monomers and at least one in-built terminal amino linker, said synthetic polysaccharide mimics natural polysaccharide obtained from gram negative bacteria such asserogroups A, C, Y, W, X andand carrier protein is obtained from gram positive bacteria such as(tetanus toxoid) or(CRM197) or their recombinant versions. The conjugation chemistry of the said oligosaccharide-protein conjugate of the present invention is thio-ether linkage. The present invention takes complete process time in the range of 14-22 hours. The said oligosaccharide-protein conjugates are useful in production of monovalent vaccine or multivalent combination vaccines and as diagnostic tool.

Process of conjugation and novel synthetic oligosaccharide-protein conjugates obtained thereof

The present invention relates to a novel process for purifying bacterial polysaccharide. It is an efficient and scalable process for removing impurities fromserogroup C (Men-C) polysaccharide which is capable of being used as such in a derivatized form or linked to other molecules, for the preparation of vaccines, more particularly conjugate vaccines forinfection.

Downstream process for purifying polysaccharides

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Delhi, India

Manoj Kumar Chhikara