Forschungszentrum Julich Gmbh

Forschungszentrum Juelich Gmbh

Manfred Biselli

Christian Wandrey

Hans-Joachim Schmoll

Joerg Thoemmes

Bernd Scr Oder

Christoph Born

Bernd Schroder

Stem cells or other organ-function cells are cultivated in a fluidized bed system on macroporous glass carrier bodies treated with a structure protein such as gelatin and an extracellular matrix protein such as fibronectin, and coated with a stroma cell layer. Glass carriers coated with gelatin are added to a fluidized bed reactor, and a culture medium containing an extracellular matrix protein is added to bind the protein to the gelatin. Stromal cells are then added and the cells are cultured to immobilize the cells on the carriers containing the bound protein. Immature organ-function cells are added to the reactor, and while generating a fluidized bed of the carriers in the culture medium, the culture medium is recirculated from and to the reactor in a recirculation loop. Bubble-free aeration of the culture medium is effected to cultivate the immature organ-function cells on the carriers to obtain both mature differentiated organ-function cells and progenitor organ-function cells. Mature differentiated organ-function cells which are released from the carriers are harvested from the reactor while retaining the progenitor organ-function cells which adhere to the carriers to provide an equilibrium between the harvested mature differentiated organ-function cells, the progenitor cells and the immature cells added to the reactor. Growth and regulatory factors for cultivating organ-function cells can be produced by culturing immobilized stromal cells in the reactor in the absence of the organ-function cells.

Culturing cells on macroporous glass carriers coated with gelatin,

A cell culture reactor can have one or more gasification modules in which tubular membrane hoses surround rigid inner tubes which extend above the membranes to communicate with gas inlets or outlets. The tube is anchored in a closure member at the lower end of the membrane.

Gasification-tube module and reactor for cell cultivation

A process for the removal of ammonium from aqueous liquids, especially from fermentation broths, is disclosed. The process uses a cation exchanger membrane which separates a liquid on a donor side that is to be depleted of NH.sub.4.sup.+ from a solution on an acceptor side whose pH is higher than on the donor side. Because of the equilibrium

Process and apparatus for removing ammonium from aqueous liquids

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Munich, Georgia

Manfred Biselli