Jin Ro Limited

Man-Keun Kim

Young-Ho Moon

Hong-Seob Jeon

Kyu-Whan Choi

Kwan-Ho Lee

Chul-Hwan Kim

Han-Seung Jeong

Byeong-Kook Na

The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide homology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918 bp of one open reading frame and termination codon; and polyadenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33 bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.

Genome coding phytolacca antiviral protein and a recombinant expression

The present invention provides a process for preparing a transgenic plant producing antiviral protein, which is transformed with a recombinant vector for Phytolacca insularis antiviral protein(PIP) expression. In accordance with the present invention, a recombinant expression vector for Phytolacca insularis antiviral protein made a grant of broad vital-resistance to transgenic plant transformed therewith, grounded on the expression of PIP.

Process for preparing virus-resistant transgenic plant

The present invention provides a novel expression vector for Phytolacca antiviral protein isolated from Phytolacca americana L. and a microorganism transformed with said PAP expression vector. In accordance with the present invention, biologically active PAP can be produced in a massive manner from the microorganism transformed with the expression vector of the invention; and, therefore, the recombinant PAP can be practically applied to various fields, e.g., the molecular studies on PAP and treatment of AIDS employing immunoconjugate.

Expression vector for Phytolacca antiviral protein

The present invention provides a recombinant vector for phytolacca antiviral protein isolated from Phytolacca americana L. and a process for preparing transgenic plants transformed therewith. The transgenic plants of the invention were properly transformed with the recombinant vector; PAP was expressed from the transgenic plants in a successful manner; and, virus proliferation was efficiently inhibited by the expressed PAP in the transgenic plants.

Expression vector for phytolacca antiviral protein and process for

The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide hornology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918 bp of one open reading frame and termination codon; and polyaclenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33 bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.

Growing community of inventors

Seoul, South Korea

Man-Keun Kim