Mitsubishi Chemical Corporation

Ajinomoto Co., Ltd.

Nec Corporation

Mitsubishi Corporation

Makoto Murase

Hiroyuki Kojima

Kenji Yamagishi

Ryusuke Aoyama

Jun Nakamura

Hiroyuki Tanaka

Mika Moriya

Keita Fukui

Chie Koseki

Jun Imamura

Kiyohiko Nishi

Junko Murase

Shuichi Yunomura

Tatsuo Nishizawa

Mari Iwabuchi

Nobuhiro Ikezawa

Daisuke Kido

Miki Ikuta

Kazunari Mizuguchi

Toshio Tsubota

Takahiko Hayakawa

Daisuke Kitahara

Akiko Sakamoto

Sanae Sato

Katsunori Yoshikawa

Madoka Yonekura

Sanae Koike

A method for producing a useful protein using a plant of the present invention comprises the steps of:

Method for producing protein in plants using lighting with at least 50% red light

Provided is a method of producing succinic acid in which a microorganism having a succinic acid-producing ability is allowed to react with a sugar, the method being characterized in that the ratio of the oxygen transfer rate to the succinic acid production rate (mmol-O/mol-SA) is 0.1 to 240 and that the doubling time of the microorganism during the reaction is not shorter than 40 hours.

Method of producing succinic acid

Provided is a bacterium which is capable of producing an organic acid and is modified so as to have an enhanced 2-oxoglutarate dehydrogenase activity as compared with that of an unmodified strain. An organic acid such as succinic acid can be produced by culturing the bacterium.

Bacterium having enhanced 2-oxoglutarate dehydrogenase activity

Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.

Method of producing succinic acid with bacterium comprising a modified fumarate reductase gene or a modified succinate dehydrogenase gene

Non-amino organic acids such as succinic acid, malic acid and fumaric acid are produced by reacting bacterial cells or treated bacterial cells of a coryneform bacterium with an organic raw material in an aqueous medium containing magnesium carbonate and/or magnesium hydroxide, and a certain range of concentration of a monovalent cation, while maintaining the pH within a certain range without increasing the volume of the aqueous medium.

Method for producing non-amino organic acid

A drive current and a regenerative current are alternately applied to a motor during a PWM (pulse width modulation) time period. The regenerative current is produced by an electromagnetic induction of an armature and flows in the opposite direction as the drive current. Therefore, a rotating speed of the motor is reduced, so that a speed-reduction following characteristic for the motor is improved. By varying the duty factor, the lengths of time during which the drive current and the regenerative current are flowing can be controlled. Thus, the speed of the motor can be controlled.

Motor drive apparatus and motor driving method capable of simply

In order to obtain a crop which provides an increased storage lipid content in its seed, the activity of cytosolic pyruvate kinase of a seed is substantially lowered as compared with the endogenous activity of pyruvate kinase in the plant seed. Preferably, the activity of phosphoenolpyruvate carboxylase is substantially lowered as compared with the endogenous activity of phosphoenolpyruvate carboxylase in the seed. Thus the storage lipid content in the plant seed is increased.

Method for increasing storage lipid content in plant seed

A linear feedback shift register comprises a shift register formed of first to (n)th flipflops cascaded in such a manner that an output of a (i)th flipflop is connected to an input of a (i+1)th flipflop, where 2.ltoreq.n and 1.ltoreq.i.ltoreq.(n-1). First to (n)th output terminals are connected to outputs of the first to (n)th flipflops, respectively, and a clock terminal connected to a clock input of each of the flipflops. First to (n-1)th multiplexors of a '1-out-of-2' type are connected at their first input to a common preset value input terminal. Second inputs of the first to (n-1)th multiplexors are connected to the outputs of the first to (n-1)th flipflops, respectively. Each of the first to (n-1)th multiplexors has a control input connected to an individual control terminal. First to (n-1)th exclusive-OR gates are cascaded in such a manner that a first input of a (n-1)th exclusive-OR gate is connected to the output of the (n)th flipflop, a first input of an (i)th exclusive-OR gate is connected to an output of an (i+1)th exclusive-OR gate, and an output of the first exclusive-OR gate is connected to an input of the first flipflop. A second input of the (i)th exclusive-OR gate is connected to an output of the (i)th multiplexor. With this arrangement, a generator polynomial generated by the linear feedback shift register can be modified by controlling the multiplexors through the individual control terminals.

Linear feedback shift register

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Yokohama, Japan

Makoto Murase