Discoverx Corporation

Mahesh Mathrubutham

Thomas S Wehrman

Keith R Olson

Chin Yee Loh

Methods and genetic constructs are provided for detecting the binding of nuclear hormone receptors to a coactivator/corepressor. The methods employ enzyme fragment complementation using fragments of &#x3b2;-galactosidase as the detection system. Cells are transformed to express the large fragment of &#x3b2;-galactosidase fused to a member of the complex with NHR for initiation of transcription and have it localized in the nucleus and to express the small fragment of &#x3b2;-galactosidase fused to the nuclear hormone receptor for binding to the member upon stimulation with a ligand.

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Cupertino, CA, United States of America

Mahesh Mathrubutham