Schering Corporation

Nanjingjinsirui Science & Technology Biology Corporation

Luquan Wang

Nicholas J Murgolo

Fang Liang Zhang

Zhuying Wang

Scott W Altmann

Michael P Graziano

Xiaowu Liu

Yun He

Chunjiao Wang

Zhibing Liu

Tianhui Xia

An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5'-UTR, ORF and 3&#x2032;-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm

We claim an isolated polypeptide having ability of binding with cholesterol. Said polypeptide is useful for investigating regulation of intestinal cholesterol absorption and cholesterol levels. Also, we claim a composition comprising said polypeptide bound to cholesterol or ezetimibe and a fusion protein comprising the polypeptide thereof.

NPC1L1 (NPC3) and methods of use thereof

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East Brunswick, NJ, United States of America

Luquan Wang