United States of America, as Represented by the Secretary of Agriculture

Luisa W Cheng

Larry H Stanker

Robert M Hnasko

Miles C Scotcher

Jeffery A McGarvey

Xiaohua He

Candace R Bever

Amatoxins (AMAs) are lethal toxins found in a variety of mushroom species. Detection methods are needed to determine the occurrence of AMAs in mushroom species, often suspected in mushroom poisonings. Provided herein are novel, sensitive monoclonal antibodies (mAbs) detection and purification techniques utilizing the mAbs that show selectivity for &#x3b1;-amanitin (&#x3b1;-AMA), &#x3b2;-amanitin (&#x3b2;-AMA) and &#x3b3;-amanitin (&#x3b3;-AMA).

High affinity monoclonal antibodies for detecting amanitins

High affinity monoclonal antibodies against Stx2 and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting Stx2 and variants thereof in a sample as well as neutralization of Shiga toxin in vivo.

High affinity monoclonal antibodies for detection of Shiga toxin 2 (STX2)

High affinity antibodies for binding epitopes of BoNT/B and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting BoNT/B in a sample.

High-affinity monoclonal antibodies for botulinum toxin type B

High affinity antibodies for binding epitopes of BoNT/Aand hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting BoNT/A in a sample.

Growing community of inventors

San Francisco, CA, United States of America

Luisa W Cheng