Hoechst Aktiengesellschaft

Forschungszentrum Julich Gmbh

Nutrinova Nutrition Specialties & Food Ingredients Gmbh

Jennewein Biotechnologie Gmbh

Lothar Elling

Maria Regina Kula

Thomas Kiy

Astrid Zervosen

Maria-Regina Kula

Stefan Jennewein

Rudiger Marquardt

Wolfgang Piepersberg

Julia Parkot

Brigitte Hoersch

Brigitte Horsch

Eric Huefner

Eric Hüfner

Ruediger Marquardt

Andreas Seiffert-Stoeriko

Wolf-Bernd Frommer

Henning Schrader

Leonie Engels

Andreas Seiffert-Storiko

Juergen Distler

Stefan Verseck

Andreas Stein

The present invention relates to nucleic acid and amino acid sequences fromserogroup O126, coding for/representing a novel alpha-1,2-fucosyltransferase. The invention also provides uses and methods for using the alpha-1,2-fucosyltransferase to generate fucosylated products, such as oligosaccharides, (glyco)proteins, or (glyco)lipids, in particular oligosaccharides found in human milk, such as 2'-fucosyllactose.

Fucosyltransferases and their applications

The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.

Nucleotide-sugar-synthesizing enzymes from nonparasitic protists

The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.

Method for increasing the gene expression of saccharose synthase

UDP-glucose pyro phosphorylase enzymes from nonparasitic protozoa

Method for the enzymatic galactosylation of monosaccharides and

The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution:

Process for the synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses

Purified saccharose-synthase, process for its production and its use

Growing community of inventors

Aachen, Germany

Lothar Elling