E.i. Dupont De Nemours and Company

Linda Jane DeCarolis

Stephen R Fahnestock

Hong Peng Wang

Qiong Cheng

Pierre E Rouviere

Tanja Maria Gruber

Lisa Diane Reiss

Richard C Ebersole

Raymond E Jackson

An acid-resistant peptide solubility tag (an 'inclusion body tag') is provided that is effective in producing peptides of interest in an insoluble form. Fusion peptide constructs comprising the inclusion body tag fused to a peptide of interest are provided. An acid cleavable peptide moiety separates the inclusion body tag from the peptide of interest so that acid hydrolysis can be used during subsequent processing steps to separate the tag from the desired peptide of interest. The present inclusion body tag's resistance to acid hydrolysis facilitates easier and cleaner separation of the peptide of interest after acid hydrolysis. Specifically, a ketosteroid isomerase-derived inclusion body tag is provided that has been engineered to be more resistant to acid hydrolysis.

Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate

Peptide tags, referred to here as inclusion body tags, are disclosed and are useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.

Peptide tags for the expression and purification of bioactive peptides

Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.

Solubility tags for the expression and purification of bioactive peptides

Methods and materials for the detection of the foot and mouth disease virus (FMDV). The methods may utilize PCR amplification, with or without an internal positive control, and appropriate primer pairs. The reagents to perform these methods can be supplied as a kit and/or in tablet form.

Sequences diagnostic for foot and mouth disease

Zein-based peptide tags, referred to here as inclusion body tags (IBTs), are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.

Zein-based peptide tags for the expression and purification of bioactive peptides

Cystatin-based peptide tags, referred to here as inclusion body tags (IBTs), are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.

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Wilmington, DE, United States of America

Linda Jane DeCarolis