Michigan State University

Leland F Velicer

Paul M Coussens

Peter Brunovskis

Hsing-Jien Kung

Robert J Isfort

A gene encoding for A antigen precursor, derived from a Avian herpesvirus, is described. The gene is preferably isolated from a Marek's disease herpesvirus virus (MDHV) and is approximately 1.8-1.9 kbp, found on a DNA fragment of about 2.35 kbp in size. The gene can be used as a building block for vaccines, directly as a diagnostic hybridization probe, to facilitate preparation of diagnostic test kits for MDHV by antigen production and subsequent antibody production, and as a potential source of regulatory elements such as signal peptides or promoters for use with other proteins.

Avian herpesvirus-A antigen precursor gene

DNA encoding glycoproteins gD, gI and gE from Marek's disease herpesvirus is described. The DNA is useful for probes to detect the DNA in the herpesvirus, for expression to produce the glycoproteins that can be used for producing the antibodies which specifically recognize the three glycoprotein antigens, and in the case of the latter two genes, for potential insertion sites for foreign genes and as possible sites for gene inactivation to attenuate MDV field isolates for vaccine purposes.

Marek's disease herpesvirus DNA segment encoding glycoproteins, gD, gI

DNA encoding glycoproteins gD, gI and gE from Marek's disease herpesvirus is described The DNA is useful for probes to detect the DNA in the herpesvirus, for expression to produce the glycoproteins that can be used for producing the antibodies which specifically recognize the three glycoprotein antigens, and in the case of the latter two genes for potential insertion sites for foreign genes and as possible sites for gene inactivation to attenuate MDV field isolates for vaccine purposes.

DNA encoding glycoproteins gD, gI and part of gE from Marek's disease herpesvirus is described. The DNA is useful for probes to detect the DNA in the herpesvirus, for expression to produce the glycoproteins can be used for producing the antibodies which specifically recognize the three glycoprotein antigens, and in the case of the latter two genes, for potential insertion sites for foreign genes. Novel glycoproteins and regulatory sequences are also disclosed.

Marek's disease herpesvirus DNA segments encoding glycoproteins, gD, gI,

Marek's disease herpesvirus DNA segments encoding glycoproteins, gD, gI

DNA encoding glycopropteins gD, gI and part of gE from Marek's disease herpesvirus is described. The DNA is useful for probes to detect the DNA in the herpesvirus, for expression to produce the glycoproteins can be used for producing the antibodies which specifically recognize the three glycoprotein antigens, and in the case of the latter two genes, for potential insertion sites for foreign genes. Novel glycoproteins and regulatory sequences are also disclosed.

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East Lansing, MI, United States of America

Leland F Velicer