Council of Scientific and Industrial Research

Kulbhushan Balwant Bastawde

Digambar Vitthal Gokhale

Ramesh Anna Joshi

Uttam Ramrao Kalkote

Rohini Ramesh Joshi

Sanjay Narayan Nene

Vithal Venkatrao Jogdand

Bhaskar Ganapatrao Gaikwad

Thottapillil Ravindranathan

Ulka Shrirang Puntambekar

Shamrao Ganapatrao Patil

Prabhakar K Ranjekar

Nutan Dattatraya Mahadik

Shamrao Ganpatrao Patil

Jayant Malhar Khire

Bhaskar Ganpatrao Patil

The present invention provides a process for the preparation of acidic lipase from microbial sources with activity at highly acidic pH, the process comprising growing sp. in a fermentation medium containing carbon and nitrogen sources along with nutrients, separating the fungal biomass and recovering the culture filtrate/broth and separating the lipase enzyme.

Process for the preparation of acidic lipase

A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial Microorganisms (NCIM), National Chemical Laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30&deg; C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.

Microbial process for the production of D(-)-N-carbamoylphenylglycine

A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial microorganisms (NCIM), National Chemical laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30.degree. C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.

The subject invention relates to an improved process for the production of alcohol which obviates the drawbacks and limitations of the hitherto known process, comprises growing a yeast in a conventional growth medium, fermenting the medium containing carbon and nitrogen source along with other conventional nutrient ingredients and fruit supplement in whole or in fractions like husk, pulp, powder of seeds and mixture thereof used at least 0.25% by weight allowing the resultant broth to ferment for at least 15 hours at temperature in the range of 15-37.degree. C.

Process for the production of alcohol

Growing community of inventors

Pune, India

Kulbhushan Balwant Bastawde