Codexis, Inc.

University of Toledo

Suganit Systems Inc.

Kripa Rao

Jie Yang

Sasidhar Varanasi

Onorato Campopiano

Ish Kumar Dhawan

Dipnath Baidyaroy

Andrew Shaw

Patricia Ann Relue

Dawei Yuan

Xiyun Zhang

Louis Clark

David Elgart

Jared L Anderson

John H Grate

Anantharam Dadi

Constance Ann Schall

Lorand Szabo

Praveen Paripati

Jungjoo Yoon

Guneet Kumar

Janos Torok

Sally Rhiannon Postlethwaite

Sally Postlethwaite

Anantharam Prasad Dadi

Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.

System for simultaneous isomerization and fermentation of sugars

The invention provides recombinant GH61 proteins obtained from, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated fromsupernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, &#x3b2;-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose

The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.

GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity

The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a &#x3b2;-glucosidase (BG) enzyme.

Multi-cellulase enzyme compositions for hydrolysis of cellulosic biomass

The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.

Endoglucanases

The present invention relates to a method for lignocellulosic conversion to sugar using an ionic liquid pretreatment for the saccharification of lignocellulosic biomass. Thus, cellulose, hemicellulose, when hydrolyzed into their sugars, can be converted to ethanol fuel through well-established fermentation technologies. These sugars also form the feedstocks for production of a variety of chemical and polymers. The complex structure of the biomass required pretreatment to enable efficient saccharification of cellulose and hemicellulose components to their constituent sugars.

Biomass pretreatment

Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have an inner of a xylose isomerase having a pH of 6 or above, and an outer region having a second enzymatic activity at an acidic pH.

Methods for fermentation of xylose and hexose sugars

Use of glycoside hydrolase 61 family proteins in processing of cellulose

A method for lignocellulose conversion to sugar with improvements in yield and rate of sugar production has been developed by using ionic liquid pretreatment. This new pretreatment strategy substantially improves the efficiency (in terms of yield and reaction rates) of saccharification of lignocellulosic biomass. Cellulose and hemicellulose, when hydrolyzed into their sugars, can be converted into ethanol fuel through well established fermentation technologies. These sugars also form the feedstocks for production of variety of chemicals and polymers. The complex structure of biomass requires proper pretreatment to enable efficient saccharification of cellulose and hemicellulose components to their constituent sugars. Current pretreatment approaches suffer from slow reaction rates of cellulose hydrolysis (by using the enzyme cellulase) and low yields.

Growing community of inventors

San Mateo, CA, United States of America

Kripa Rao