Chugai Seiyaku Kabushiki Kaisha

Other

Kou-ichi Jishage

Tatsuhiko Kodama

Yoshiki Yamada

Nobuo Kamada

Hideki Adachi

Naohiro Yabuta

Kiyoshi Habu

Hiroyuki Arai

Keizo Inoue

Hiroshi Suzuki

Jun-Ichi Nezu

Asuka Ose

Makoto Arita

Dieter E Jenne

A non-human animal that produces human tissue factor (TF) without substantially producing non-human animal tissue factor, said animal having a genome in which cDNA encoding human TF has been inserted upstream of the translation initiation codon for the non-human animal genomic TF gene.

Methods of using human tissue factor-producing knock-in mice

This invention provides methods for producing antibodies, wherein the methods comprise the step of administering an immunogen comprising both a target antigen and a background antigen to transgenic animals, into which a gene coding for the background antigen has been introduced. Since immunotolerance to the background antigens have thus been induced in the transgenic animals, the animals efficiently produce antibodies to target antigens.

Methods for producing antibodies

Membrane proteins that are background antigens were solubilized, and transgenic animals were produced using genes encoding these soluble proteins. Antibodies against the background antigen membrane proteins comprised in the immunogens were not found in these transgenic animals, and even when genes encoding soluble proteins were used, immunotolerance against the full-length membrane proteins could be induced. Moreover, by expressing the background antigen membrane proteins as soluble proteins inside the bodies of transgenic animals, unfavorable phenotypes that appear when the full-length membrane proteins are expressed could be avoided, and such animals were made widely available as immunized animals.

Transgenic mice expressing baculovirus soluble GP64 and methods of using such mice to make antibodies

Methods for producing antibody

Knock-in non-human animal producing human tissue factor

The present invention provides a knockout animal artificially modified to inhibit &#x3b1;-TTP gene expression. This animal is useful as a tool for understanding mechanisms for the development of familial isolated vitamin E deficiency and other diseases induced by oxidative stress (e.g., arteriosclerosis, diabetes). It is also useful as a tool for developing a therapeutic agent for these diseases.

&#x3b1;-tocopherol transport protein knockout animal

A mammal is provided, in which the LKB1 gene can be deleted phase-specifically and tissue-specifically. These mammals are highly useful as tools to reveal the onset mechanism for diseases caused by LKB1 gene deficiency, such as Peutz-Jeghers syndrome and cancers, as well as to develop therapeutic agents, methods, and so on for the diseases.

LKB1 gene knockout mice

Growing community of inventors

Shizuoka, Japan

Kou-ichi Jishage