Ajinomoto Co., Ltd.

University of Minnesota

Konosuke Sano

Kiyoshi Miwa

Shigeru Nakamori

Kazuhiko Matsui

Masaaki Ishida

Koji Mitsugi

Koichi Ito

Hiroyuki Kojima

Takayasu Tsuchida

Naohiko Yasuda

Yuri Ogawa

Keizo Matsuda

Fumihide Tamura

Hiroshi Takagi

Kazuhiko Yamada

Kazue Kawamura

Haruo Momose

Mahito Terabe

Ichiro Noda

Kazushi Togo

Kenzo Yokozeki

Hisao Ito

Osamu Kurahashi

Chikahiko Eguchi

Hidetsugu Nakazawa

Hitoshi Enei

Katsuaki Sato

Richard S Hanson

Frederick J Schendel

Chieko Osumi

Takashi Tanaka

Takaaki Sato

Shigeru Makamori

Teruhiko Kagawa

Takehiko Ichikawa

Richard Dillingham

A method of producing glutamic acid by culturing a biologically pure wild type Bacillus methanolicus which exhibits sustained growth at 50.degree. C. using methanol as a carbon and energy source and requiring vitamin B.sub.12 and biotin is provided.

Production of glutamate using wild type Bacillus methanolicus

Method of producing L-lysine by fermentation

Method of process for producing L-lysine by fermentation

Method for the production of L-threonine by fermentation, using mutated

A method for producing L-tryptophan comprising culturing coryneform glutamic acid-producing bacteria transformed with an expression vector capable of replicating and expressing an operably linked anthranilic acid phosphoribosyl transferase gene isolated from a brevibacterium glutamic acid-producing bacterium, growing the transformants in culture medium allowing for the production of L-tryptophan and recovering the L-tryptophan accumulated in the culture medium.

Method for producing L-tryptophan by fermentation

A brevibacterium containing a recombinant DNA molecule constructed by operatively connecting a gene coding for shikimate kinase isolated from Brevibacterium lactofermentum to a plasmid vector capable of replicating in the Brevibacterium is disclosed along with methods of preparing this bacterium and of using it in the production of aromatic amino acids.

Coryneform bacteria carrying recombinant DNA and process for producing

A method for producing an L-amino acid, which comprises inserting a gene which codes for an enzyme which is utilized on the route of biosynthesis of an L-amino acid product into one of at least two plasmid vectors which have compatible replicating origins different from each other, inserting a second gene which codes for an enzyme different from the first enzyme on the route of biosynthesis of the L-amino acid into a second of the plasmid vectors; introducing the thus obtained recombinant plasmids into a strain of Coryneform bacteria; and culturing the thus transformed strain which is capable of producing the L-amino acid, said two enzymes being highly rate determining enzymes for the biosynthesis of L-amino acid.

Method for producing L-amino acids

The present invention relates to a process for producing an aromatic amino acid which comprises culturing a Coryneform bacterium carrying a recombinant DNA constructed by connecting (1) a gene capable of being expressed in a Coryneform bacterial cell and coding for 3-deoxy-D-arabino-heptulonic acid-7-phosphate synthetase with (2) a plasmid vector capable of propagating in a Coryneform bacterial cell.

Coryneform bacteria carrying recombinant DNA and a process for producing

A recombinant DNA molecule comprising a plasmid vector having operationally inserted therein a gene coding for homoserine kinase is disclosed along with bacteria containing this recombinant DNA molecule and methods of using these bacteria to produce amino acids in large quantities.

Recombinant DNA, bacteria carrying said recombinant DNA and a process

A process for producing L-tryptophan in which a suitable substrate such as a carbohydrate, indole or anthranilic acid is contacted with Coryneform bacteria, where the Coryneform bacteria bear recombinant DNA constructed by connecting a gene coding for tryptophan synthetase with a plasmid vector capable of proliferating in Coryneform bacteria.

Process for producing L-tryptophan

A genetic sequence coding for the production of a protein having the activity of diaminopimelic acid decarboxylase and having two Pst I cleavage sites in its DNA chain and a molecular weight of 2.9.+-.0.05 Md, is incorporated into a vehicle capable of replication in Coryneform bacteria and used to produce L-lysine by fermentation.

Coryneform bacteria carrying recombinant plasmids and their use in the

A composite vector capable of the transduction of a Coryneform bacterium, which comprises a first DNA segment containing at least the genetic information for replication from a plasmid capable of propagating in a Coryneform bacterium and a second DNA segment containing at least a DNA region of a phage capable of propagating in the Coryneform bacterium, wherein the DNA region is incorporated into a particle of the aforementioned phage when the aforementioned bacterium is infected with the phage, is disclosed.

Transducible composite plasmid

A substantially pure plasmid which is characterized by a molecular weight of 3.0.+-.0.1 megadalton and the restriction endonuclease-cleavage map shown in the Figure. This plasmid is capable of propagating in Corynebacteria and due to its size is useful as a vector for the cloning of exogenous genes.

Plasmid

A recombinant DNA molecule comprising a plasmid vector having operationally inserted therein a gene coding for phosphoenol pyruvate carboxylase is disclosed along with bacteria containing this recombinant DNA molecule and methods of using these bacteria to produce amino acids in large quantities.

Recombinant DNA having a phosphoenol pyruvate carboxylase gene inserted

A genetic sequence coding for the production of a protein having the activity of homoserine dehydrogenase and having two Pst I cleavage sites in its DNA chain and a molecular weight of 2.24 Md, is incorporated into a vehicle capable of replication in Coryneform bacteria and used to produce L-threonine and L-isoleucine by fermentation.

Growing community of inventors

Tokyo, Japan

Konosuke Sano