Stellenbosch University

Lallemand Hungary Liquidity Management LLC

Danstar Ferment Ag

Universiteit Stellenbosch

Kevin S Wenger

Aaron Argyros

Erin Wiswall

Ryan Skinner

Trisha Barrett

Nicky Caiazza

John E McBride

Charles F Rice

Allan C Froehlich

Mark Mellon

Anne K Warner

Riaan Den Haan

Emily Stonehouse

Shital A Tripathi

Haowen Xu

Chhayal V Gandhi

Kristen M Deleault

John S Bardsley

Frank Agbogbo

Indraneel Shikhare

Alan Belcher

Vineet B Rajgarhia

Abigail Foster

Jennifer Gosselin

Yin-Ying Chiu

Elena E Brevnova

Heidi H Hau

Brooks Henningsen

Janet Fisher

Michelle Oeser

J Kevin Kraus

Johannes Van Eijk

Heidi Lau

Elena Brevnova

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

Cell-associated heterologous food and/or feed enzymes

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

Growing community of inventors

Hanover, NH, United States of America

Kevin S Wenger