The Green Cross Corporation

Ken Okabayashi

Haruhide Kawabe

Kiyoshi Tsutsui

Yukimitsu Nakagawa

Kazuo Ikegaya

Yutaka Ishida

Kohji Murakami

Hitoshi Minamino

Sadao Ueda

Naofumi Hayasuke

Kazumasa Yokoyama

Kaoru Kobayashi

Takao Ohmura

Hirofumi Arimura

Masami Miura

Hideyuki Oi

Kazumoto Hirabayashi

Miho Shimizu

Atsushi Masaki

Atusi Masaki

A plasmid for a yeast host, the plasmid comprising in sequence: (1) a yeast derived promoter, (2) an albumin-encoding region placed under control of the yeast-derived promoter, (3) a transcription terminator and (4) a sequence homologous to a part of the yeast host chromosomal sequence such that the plasmid is capable of being integrated into the yeast host cell chromosome and wherein the plasmid is incapable of autonomous replication in yeast cells; a yeast host transformed with the above plasmid; a method of producing a yeast transformant which comprises transforming a yeast host by integrating at least two different plasmids each having a sequence homologous to a part of the host yeast chromosomal sequence one at a time into the chromosome of the yeast host cell; and a method of producing albumin which comprises cultivating the above transformant and recovering the thus-produced albumin.

Albumin gene-containing plasmid, transformant carrying same, production

A methylotrophic and glucotrophic mutant strain capable of producing a heterologous protein and a method for producing a heterologous protein, comprising culture of the mutant strain. The mutant strain of the present invention can be grown in a medium containing both methanol and glucose, with the effect that the growth of the strain and production of a heterologous protein proceed at the same time. Accordingly, a heterologous protein can be produced in a large amount in a short time.

Mutant strain of Pichia pastoris which utilizes methanol in the presence

A method for preparing foreign protein in yeast using an expression recombinant DNA comprising DNA encoding the serum albumin signal peptide adjacent to DNA encoding the foreign protein is disclosed.

Method of preparing foreign protein in yeast, recombinant DNA,

Novel signal peptides capable of functioning in yeasts have the amino acid sequence:

DNA's encoding signal peptides

Growing community of inventors

Osaka, Japan

Ken Okabayashi