Ortho-Clinical Diagnostics, Inc

Sigma-Aldrich Co. LLC

Keming Song

Brian W Ward

Jeffrey M Linnen

David R Patterson

Stephanie Uder

Chuan Li

Kevin M Gorman

Ron Hernan

John A Puskas

Leanne M Snyder

Kristen E Bettinger

Kristen E Opper

Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.

Processes for directionally ligating double stranded nucleic acids

Recombinant DNA processes using a dNTP mixture containing modified nucleotides

Disclosed herein are methods and kits for the simultaneous detection of hepatitis C virus and human immunodeficiency virus in biological samples from human subjects.

Oligonucleotide primers for efficient multiplex detection of hepatitis C virus (HCV) and human immunodeficiency virus (HIV) and methods of use thereof

Disclosed herein are methods and kits for the detection of human immunodeficiency virus in biological samples from human subjects. Oligonucleotide reverse transcription primers for use in such methods and kits for detection of human immunodeficiency virus are also described.

Oligonucleotide reverse transcription primers for efficient detection of HIV-1 and HIV-2 and methods of use thereof

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Ballwin, MO, United States of America

Keming Song