Ajinomoto Co., Ltd.

Mitsubishi Chemical Corporation

Keita Fukui

Miku Toyazaki

Sayaka Asari

Hiroyuki Kojima

Benjamin Mijts

Jun Nakamura

Christine Roche

Yoshihiko Hara

Yoshinori Tajima

Yoshihiro Usuda

Kazuhiko Matsui

Kazue Kawamura

Chie Koseki

Keiko Danjo

Peter Kelly

Seiko Hirano

Kenji Yamagishi

Kenichi Hashiguchi

Makoto Murase

Akito Chinen

Kazuyuki Hayashi

Yasuaki Takakura

Yoko Mihara

Ryusuke Aoyama

Keiko Noguchi

Shuichi Yunomura

Daiki Yahagi

Madoka Yonekura

Suguru Yamakami

Rekha Murali

Hitoshi Takayashiki

Takuto Ono

Junko Ito

Matthew Davis

Akiko Sakamoto

Sanae Sato

A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism that is able to produce the objective substance, which microorganism has been modified so that the activity of an enzyme involved in SAM cycle (SAM cycle enzyme) is increased.

Method for producing objective substance

A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of S-adenosyl-L-homocysteine hydrolase is increased.

A method is described for producing an objective substance, for example, an aldehyde such as vanillin. The objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an ability to produce the objective substance, wherein the microorganism has been modified to have a specific carboxylic acid reductase (CAR) gene, such as aCAR gene,CAR gene, orCAR gene.

Method for producing aldehyde

A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of enolase is reduced.

A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so as to have a specific feature, such as a reduced activity of AICAR formyltransferase/IMP cyclohydrolase, an increased activity of 3-PGDH, and/or a reduced activity of L-serine deaminase.

A method for producing benzaldehyde is provided. Benzaldehyde is produced from L-phenylalanine or a carbon source by using microorganism(s) having amino acid deaminase, 4-hydroxymandelate synthase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase.

Method for producing benzaldehyde

A method is provided for producing an objective substance, for example, an aromatic aldehyde such as vanillin. The objective substance is produced from a carbon source or a precursor of the objective substance by using a coryneform bacterium that is able to produce the objective substance, wherein bacterium has been modified so that the activity of alcohol dehydrogenase is reduced.

A method for producing an objective substance is provided. An objective substance is produced by culturing a microorganism which has been modified so that the activity of a dicarboxylic acid exporter protein is reduced in a medium, and collecting the objective substance from the medium.

Method for manufacturing useful substance

A method for producing a dicarboxylic acid is provided. A dicarboxylic acid is produced by culturing a bacterium having a dicarboxylic acid-producing ability, which has been modified so that the expression of one or more of the yeeA gene, ynfM gene, yjjP gene, and yjjB gene is increased, in a medium, and collecting the dicarboxylic acid from the medium.

Method for producing dicarboxylic acid

A method for producing an L-amino acid of glutamate family such as L-glutamic acid is provided. An L-amino acid of glutamate family is produced by culturing a coryneform bacterium having an ability for producing an L-amino acid of glutamate family, which has been modified so that the activity of an &#x3b1;-ketoglutaric acid (&#x3b1;-KG) uptake carrier is increased, in a medium, and collecting the L-amino acid of glutamate family from the medium.

Method for producing L-amino acid of glutamate family

An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein

An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of the sucE1 and mdh genes are enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas, and collecting the organic acid.

Method for producing an organic acid

Coryneform bacteria are described that have an ability to produce L-amino acids and are modified so that acetyl-CoA hydrolase activity is decreased. The bacteria are used to produce L-amino acids generated by a biosynthetic pathway in which pyruvic acid is an intermediate, such as L-glutamic acid, L-arginine, L-glutamine, L-proline, L-alanine, L-valine, and L-lysine.

L-amino acid-producing bacterium and a method for producing L-amino acid

An organic acid is produced by allowing a bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an organic acid and has been modified so that the phosphoenolpyruvate carboxykinase activity is enhanced, which is selected fromandbacteria, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.

An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.

Growing community of inventors

Kanagawa, Japan

Keita Fukui