Morinaga Milk Industry Co., Ltd.

The Green Cross Corporation

The Japan Tobacco Salt Public Corporation

Katsuhiro Ogasa

Morio Kuboyama

Nobuya Yanai

Minoru Saito

Mamoru Tomita

Muneo Yamada

Fumimaro Takaku

Masayuki Nishida

Tsutomu Kudo

Kunisuke Kuwahara

Eiji Takahashi

Hiroshi Shimada

Satoshi Funakoshi

Seiichi Shimamura

Shigeo Okonogi

Teruhiko Mizota

Kazuo Maeda

Hiroya Yuguchi

Kazuhiro Nagata

Ryo Kato

Yusuke Miyazaki

Joji Ono

Kazuyoshi Doi

Yoshitsugu Harada

Katsuichi Noguchi

Akio Kawashiri

Yoshinobu Hayashi

Katsuto Okada

Hiroshi Miyagawa

Rye Kato

Yoshiteru Watanabe

A colony stimulating factor effective in treating human granulocytopenia is produced by cultivating monocytes and macrophages isolated from the human peripheral blood in a synthetic medium for tissue culture containing a glycoprotein isolated from human urine and capable of stimulating the formation of human granulocytes or mouse macrophages and granulocytes.

Method for producing substance capable of stimulating differentiation

A method of manufacturing a packaged soybean curd with a long shelf life without the inclusion of any artificial additives such as coagulating agents, germicides and the like; wherein soybean juice is subjected to lactic acid fermentation until its pH is reached to a value equal to or less than a value which is determined from the percentage of the solids content (5-16 wt. %) of soybean juice and then is subjected to heating (60.degree.-95.degree. C., 10-100 minutes) to adjust the curd tension to above 20 g.

Method of manufacturing a packaged soybean curd

A colony-stimulating factor having definite physical and chemical properties and a function of stimulating activity on human bone marrow cells to proliferate and differentiate, thereby forming granulocyte colonies, is obtained from human urine by concentrating the urine with respect to proteins contained therein by adsorption chromatography with silica gel, salting out with ammonium sulfate and other means, then removing impurities by adsorption on cation exchanger, and further purifying by ion exchanging chromatography on anion exchanger, gel filtrating chromatography with highly crosslinked gels, affinitive chromatography with sugar affinitire adsorbents and electrophoresis.

HGI-Glycoprotein capable of stimulating proliferation and

A colony-stimulating factor having definite physical and chemical properties and a function of stimulating activity on human bone marrow cells to proliferate and differentiate, thereby forming granulocyte colonies, is obtained from human urine by concentrating the urine with respect to proteins contained therein by adsorption chromatography with silica gel, salting out with ammonium sulfate and other means, then removing impurities by adsorption on cation exchanger, and further purifying by ion exchanging chromatography on anion exchanger, gel filtrating chromatography with highly crosslinked gels, affinity chromatography with sugar affinitive adsorbents and electrophoresis. This substance is stable in the presence of a stabilizer such as albumin or urinary proteins, against heat-treatment of virus-inactivation and can be used as a leukopenia curative which is precluded from fear of virus-infection.

Virus-inactivated HGI-glycoprotein capable of stimulating proliferation

Latex reagents for quantitative assay of a variety of steroid hormones or metabolites thereof which are contained in body fluid or excreted fluid of human beings comprise latex particles which are immunologically sensitized with a conjugate selected from a variety of steroid-serum albumin conjugates in which the steroid to be detected is bonded to serum albumin in a very small ratio ranging from 0.5 to 7 molecules per 1 molecule of the serum albumin used. Sensitization of latex particles with such a conjugate is performed using a limited amount dependent on the bonding ratio of steroid molecules per molecule of serum albumin in the conjugate and the particle size of the latex used. The latex reagents show very high sensitivity amounting to 0.2-0.04 nmole steroid equivalent/ml.

Latex polymer sensitized with steroid-serum albumin conjugate,

An orally administered powdery composition comprising freeze dried viable cell mass of the genus Bifidobacterium and powdered lactulose is effective for the establishment of favorable Bifidobacteria in cases of abnormal intestinal flora. The composition contains viable cell mass of the genus Bifidobacterium, has a high cell density not less than 8.times.10.sup.10 per gram, and exhibits a high cell survival rate over a long period of storage.

Powdery composition comprising viable Bifidobacteria cells and lactulose

Method for manufacturing a lactulose powder with a low hygroscopicity by mixing ethyl alcohol to a highly hygroscopic lactulose-containing powder followed by allowing the resulting mixture to stand or stirring it for at least 1 hour, separating the lactulose-containing material insoluble in ethyl alcohol from the mixture and removing ethyl alcohol from the material separated.

Method for preparing a non-hygroscopic lactulose-containing powder

A method for manufacturing rough textured soya bean curd of long shelf life from conventional soya bean juice comprises the steps of heat-sterilizing the soya bean juice, adding a coagulant to the sterilized soya bean juice to form coagulum, adjusting the water content of the coagulum to 78% to 90% by weight, packing the adjusted coagulum into a container followed by hermetically sealing and shaping the coagulum by heating.

Method for manufacturing rough textured soya bean curd

A process for preparing a therapeutic agent characterized by preculturing human amnion cells isolated from human amnion by trypsinization or human amnion cells obtained by subculturing the isolated cells in a medium containing calf or bovine fetal serum at least one time for 1 to 5 days to form a monolayer, removing the medium, adding a medium containing 0.5 to 20 mg of human serum albumin per 1 ml of medium thereto and culturing it for 3 to 6 days, dialyzing the culture liquid against diluted buffer or water, and filtering the dialyzed culture liquid through a bacterial filter thereby obtaining a germ free liquid agent.

Process for preparing a curing agent for myelogenic leukemia

A process for preparing a free-flowing lactulose-containing powder from a solution containing lactose, by adding calcium hydroxide to the solution to adjust the pH of said solution to from 9.4 to 11.2; heating the resulting solution so that the pH is reduced to from 7.5 to 9.0; the homogenizing, concentrating and drying.

Process for preparing a lactulose-containing powder for feed

A granular composition having a grain size of 10 to 50 mesh and a bulk density of 0.5 to 0.7 g/cm.sup.3 suitable for use in a tobacco filter or a tobacco pipe and having the ability to selectively remove carcinogenic and deleterious components from tobacco smoke comprises granules which are composed of bovine milk whey protein power and/or egg white protein powder, and one or more excipients selected from the group consisting of powdered cellulose, wheat flour, starch, rice flour, sugar, glucose, lactose, talc, alumina, zeolite or silica gel; or composite granules which are a blend of said granules and charcoal granules.

Granular composition for tobacco filter

A method of determining the viable bacterial count of food which comprises incubating said food in a liquified agar medium which contains from 0.005 to 0.1 gram per liter of said liquified agar medium 2, 3, 5-triphenyl tetrazolium chloride as an inhibitor of bacteria colony spreading, and counting the bacteria colonies.

Method for determining the viable bacteria count of food and a medium

A whipped-cream topping dessert is prepared by a process comprising the steps of: (a) formulating an aqueous mixture containing 10 to 30 percent by weight of fat, 3 to 6 percent by weight of milk protein, 12 to 22 percent by weight of saccharide, 0.3 to 0.8 percent by weight of emulsifier, 0.05 to 0.15 percent by weight of thickening agent and 0.3 to 0.6 percent by weight of gelling agent; (b) homogenizing said mixture; (c) pasteurizing and cooling the mixture to room temperature; (d) thereafter adding an aqueous solution of a calcium salt to said mixture so that the amount of calcium ion in said mixture is 10 to 35 mg. per 1 gram of the milk protein; (e) whipping and heating the resultant mixture at a temperature of 25.degree. to 30.degree.C to prepare a flowable whipped-cream; (f) topping said whipped-cream onto a lower layer dessert base maintained at a temperature of 50.degree. to 70.degree.C which has previously been placed into a container; and (g) solidifying both layers by cooling.

Growing community of inventors

Yokohama, Japan

Katsuhiro Ogasa