Massachusetts Institute of Technology

Aventis Pharmaceuticals Inc.

Katherine M Call

Daniel Arie Haber

David E Housman

Alan J Buckler

Thomas M Glaser

Caryn Y Ito

Jerry M Pelletier

Elise A Rose

Wendy Bruening

Andre Darveau

Paul R August

Pamela J Keagle

Henry Long

Anna Wiencis Montmayeur

André Darveau

Wendy Breuning

The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.

Transposon mediated multiplex sequencing

The Wilms' tumor gene associated with 11p3 locus on the human chromosome, as well as a method of analyzing cells for the gene is described and characterized. The gene encodes a transcription unit approximately 50 kb in size and a mRNA of approximately 3 kb, which is expressed in predominantly in kidney and gonadal tissue. The gene is alternative spliced producing four very similar mRNA transcripts. The polypeptides encoded by the Wilms' tumor DNA includes four 'zinc fingers' and a region rich in proline and glutamine, suggesting that the polypeptide has a role in transcription regulation.

Localization and characterization of the Wilms' tumor gene

The Wilms' tumor gene associated with 11p3 locus on the human chromosome, as well as a method of analyzing cells for the gene is described and characterized. The gene encodes a transcription unit approximately 50 kb in size and a mRNA of approximately 3 kb, which is expressed in predominantly in kidney and gonadal tissue. The gene is alternative spliced producing four very similar mRNA transcripts. The polypeptides encoded by the Wilms' tumor DNA includes four &ldquo;zinc fingers&rdquo; and a region rich in proline and glutamine, suggesting that the polypeptide has a role in transcription regulation.

The Wilms' tumor gene associated with the 11p13 locus on the human chromosome, as well as a method of analyzing cells for the gene is described and characterized. The gene encodes a transcription unit approximately 50 kb in size and a mRNA of approximately 3 kb, which is expressed in a limited number of cell types (e.g., predominantly kidney cells and ja subset of hematopoietic cells). The polypeptide encoded by the Wilms' tumor DNA includes four 'zinc fingers' and a region rich in proline and glutamine, suggesting that the polypeptide has a role in transcription regulation.

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Malden, MA, United States of America

Katherine M Call