Cargill Inc.

Natureworks LLC

Teknologian Tutkimuskeskus Vtt

Danisco Sweeteners Oy

Kari Koivuranta

Laura Ruohonen

Aristos Aristidou

Pirkko Suominen

Vineet Rajgarhia

Merja Penttilä

Marja Ilmen

Christopher K Miller

Stacey Olson

Merja Elisa Penttila

Kevin Roberg-Perez

Brian Jeffrey Rush

Catherine Asleson Dundon

Thomas W McMullin

Benjamin Matthew Hause

Marja Ilmén

John Londesborough

Heikki Ojamo

Peter Richard

Chris Miller

Mervi Toivari

Hakan Gros

Merja Pentilla

Andrei Miasnikov

Josef Deutscher

Mira Povelainen

Claire Plazanet-Menut

Pirrko Suominen

Thomas William McMULLIN

Merja Penttilae

Merja Pentillä

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the generaor. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

Fermentation process using yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species, and fermentation processes using genetically modified yeast

The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phospholipid: diacylglycerol acyltransferase (PDAT) activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinantcell and its construction is described.

Genetically modified fungi and their use in lipid production

Cells of the speciesand closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.

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Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species and fermentation processes using genetically modified yeast

The invention relates to the methods of manufacturing five-carbon sugars and sugar alcohols as well as other compounds derived from pentose-phosphate pathway from readily available substrates such a hexoses using metabolically engineered microbial hosts.

Manufacture of five-carbon sugars and sugar alcohols

The present invention relates to biocatalysts that are cells, optimally of the Crabtree-negative phenotype, comprising expression vectors encoding genes heterologous to the cell that enable increased production of organic products. More specifically, the invention relates to genetically modifiedcells, methods for making thecells, and their use in production of organic products, particularly lactic acid.

This invention provides biocatalysts that are recombinant yeast cells comprising recombinant expression vectors encoding heterologous lactate dehydrogenase genes for producing lactate.

Methods and materials for the synthesis of organic products

Growing community of inventors

Helsinki, Finland

Kari Koivuranta