Boston University

Massachusetts Institute of Technology

Abbott Gmbh & Co Kg

Karen Nazaretian Allen

Mark W Grinstaff

Lee Daniel Arnold

Richard W Dixon

Barbara Imperiali

Hans Wolfgang Hoeffken

James Galagan

Uros Kuzmanovic

Mingfu Chen

Nancy J Bump

Cornelia Bellamacina

Katherine J Franz

Margarita Alexandrovna Tararina

Described herein is an amperometric biosensor, e.g., chronoamperometric biosensor for the measurement of the concentration of nicotine. Also disclosed herein is a wearable nicotine biosensor device and a biosensor that detects nicotine in smoke. The biosensor disclosed herein comprises a nicotine-catalyzing enzyme, such as NicA2 or mutant NicA2 enzymes. Also described herein are systems comprising said amperometric biosensor, e.g., chronoamperometric biosensor and methods of using said chronoamperometric biosensor.

Enzyme-based electrochemical nicotine biosensor

The present invention relates to polypeptides which comprise the ligand binding domain of Tie-2, crystalline forms of these polypeptides and the use of these crystalline forms to determine the three dimensional structure of the catalytic domain of Tie-2. The invention also relates to the use of the three dimensional structure of the Tie-2 catalytic domain both alone, or in complex with inhibitors, in methods of designing and/or identifying potential inhibitors of Tie-2 activity, for example, compounds which inhibit the binding of a native substrate to the Tie-2 catalytic domain.

Method of identifying inhibitors of Tie-2

The present invention provides lanthanide binding tags (LBT) that selectively complex trivalent lanthanide (Ln) ions and afford stable complexes with desirable physical properties, including at least one of fluorescence and anomalous x-ray scattering.

Lanthanide binding tags

Growing community of inventors

Weston, MA, United States of America

Karen Nazaretian Allen