Ceskoslovenska Akademie Ved

Karel Blaha

Jiri Coupek

Jaroslava Turkova

Olga Valentova

The invention relates to specific sorbents based on hydrophylic polymeric gels, preferably of a macroporous character, which carry covalently bonded D-aminoacid or peptide which contain D-aminoacid units. The basic carrier prepared by copolymerization of hydroxyalkyl esters or hydroxyalkylamides of acrylic and methacrylic acid with crosslinking acrylate or methacrylate comonomers are modified by the reaction with diamines, aminoacids or dicarboxylic acids and the resulting carboxyterminal or aminoterminal groups are condensed with D-analogs of aminoacids or peptides. The peptide containing D-aminoacids can be also stepwise synthetized on the surface of the carrier. The specific sorbents are further used as insolubilized inhibitors in the affinity chromatography of proteolytic enzymes. They do not undergo an irreversible inactivation during repeated application and are suitable for technological purposes because of their mechanical and hydrolytical stability.

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Prague, Czechia

Karel Blaha