Bayer Aktiengesellschaft

Jurgen Distler

Wolfgang Piepersberg

Ansgar Stratmann

Anneliese Crueger

Claude Taranta

Tatjana Sikuljak

Simon Nord

Hans-Georg Dellweg

Richard A Warriner

Thomas Bork

Werner Schroder

Britta Katz

Daniel Bihlmeyer

Jurgen Georg Lenz

James Thomas Wofford

Klaus Goeke

Hermann Pape

Michael Hemker

Beate Schaper

Tina Schroder-Grimonpont

The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.

Processes for preparing acarviosyl transferase and for using it in the

The invention relates to acarbose biosynthesis genes from actinomycetes, predominantly from Actinoplanes sp. SE 50/110 and its mutants, to a process for the isolation of acarbose biosynthesis genes from actinomycetes using a gene probe which has been derived from highly conserved protein regions of known dTDP-glucose dehydratase enzymes for finding the genes acbA (coding for dTDP-glucose synthase), acbB (coding for dTDP-glucose dehydratase) and acbC (coding for a cyclase, in part identical to AroB, bacterial 3-dehydroquinate synthases) or one or more acarbose biosynthesis genes from Actinoplanes sp., and to the use of the acarbose biosynthesis genes.

Acarbose biosynthesis genes from actinoplanes sp., process for the

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Wuppertal, Germany

Jurgen Distler