Hitachi, Ltd.

Joled Inc.

Yuki Gosei Kogyo Co., Ltd.

Microsoft Technology Licensing, LLC

Japan Tobacco Inc.

Junko Tanaka

Tatsuo Nakagawa

Yuzuru Shimazaki

Takahiro Komatsu

Kunio Harada

Kosuke Mishima

Kazuo Itoh

Hiroshi Katagiri

Junji Ohki

Nobuhiro Mizutani

Kiyoo Itoh

Jin Hee Jeon

Hiromasa Noda

Masao Kamahori

Chihiro Uematsu

Hajime Matsushita

Makoto Yamagishi

Takashi Ebata

Takahide Yokoi

Yoshinobu Kohara

Sigeo Ihara

Shin'ichiro Kimura

Yoshiyuki Ota

Toru Toyabe

Ryogo Kanatani

Daniel J Moffitt

Hirokazu Sawada

Yasushi Gotoh

Junko Kyomasu

Shigeki Higuchi

Yu Kuratake

Hiromi Kaibara

Fumiyuki Maeyama

Akiko Shiratori

Nobuko Miwa

Yasufumo Shiraishi

Sachio Kono

The present disclosure provides a technique capable of manufacturing a measurement cell having a high specimen utilization ratio in the case of using a measurement cell that introduces a specimen into a surface hole. In the measurement cell manufacturing method according to the present disclosure, a measurement cell includes a channel wall protruding from the lower surface substrate toward the through-hole, and a specimen solution is introduced into a lower surface side space to introduce the specimen into the through-hole.

Measurement cell manufacturing method and measurement cell

A digital PCR measuring apparatus capable of measuring a melting curve with high accuracy is provided. The digital PCR measuring apparatus includes a temperature adjuster that controls a temperature of a sample container including a plurality of minute regions, a fluorescence measurement part that measures fluorescence intensity of a plurality of minute regions, and a controller that controls the temperature adjuster and the fluorescence measurement part. The controller controls the temperature adjuster to raise a temperature of the sample container, and, after removing air bubbles generated in the sample container, measures fluorescence intensity of a plurality of minute regions while controlling the temperature adjuster to lower the temperature of the sample container, and measures a melting curve of a plurality of the minute regions.

Digital PCR measuring device

The present invention addresses the problem of providing a novel digital PCR analysis method. One embodiment of the novel digital PCR analysis method is a DNA detection method including the steps of: partitioning a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a target DNA to be detected into a plurality of compartments; carrying out a nucleic acid amplification reaction in the compartments; measuring a fluorescence intensity in association with a temperature change; calculating a melting temperature of a DNA double strand based on a change in the fluorescence intensity in association with the temperature change; and calculating a ratio of a fluorescence intensity at a second temperature that is lower than a first temperature in association with the temperature change to that at the first temperature.

Method and device for digital PCR measurement

The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

PCR measuring method and measurement device

An organic electroluminescence (EL) element including an anode, a light-emitting layer above the anode, a first functional layer on and in contact with the light-emitting layer, a second functional layer on and in contact with the first functional layer, and a cathode above the second functional layer. A lowest unoccupied molecular orbital (LUMO) level of the first functional layer is lower than at least one of a LUMO level of the second functional layer and a Fermi level of a metal material included in the second functional layer.

Organic EL element and organic EL display panel

An organic electroluminescence (EL) element including an anode, a cathode opposing the anode, a light-emitting layer between the anode and the cathode, a hole transport layer in contact with the light-emitting layer, between the light-emitting layer and the anode, and an electron transport layer in contact with the light-emitting layer, between the light-emitting layer and the cathode. A difference between a lowest unoccupied molecular orbital (LUMO) level of an organic material included in the light-emitting layer and a LUMO level of an organic material included in the electron transport layer is greater than a difference between a highest occupied molecular orbital (HOMO) level of an organic material included in the hole transport layer and a HOMO level of the organic material included in the light-emitting layer.

Organic EL element

A solution monitoring system is provided for gathering, filtering, and analyzing information associated with solutions for managing monitoring, upgrades, and migrations. Telemetry may be recorded for various types of solution events and various properties of solutions as part of an add-in intelligence. Furthermore, add-in management tools are provided for administrators and end users.

Solution monitoring system

A finely structured grooved gate transistor of which the threshold voltage does not decrease in spite of the small size and of which the threshold voltage of the transistor can be adjusted by shape. The shape of a groove corner of the transistor as a semiconductor device is contained in a concentric circle having a radius of curvature r.+-.L/5 (L: channel length) and the radius of curvature r, i.e., the geometric parameter has a relationship with the doping concentration as shown in FIG. 1B. Alternatively, the average (a+b)/2 (geometric parameter) of the sum of the two sides opposite the right angle of a right triangle formed of a straight line in contact with the gate bottom in parallel to the substrate surface of a grooved gate transistor, a perpendicular line to the substrate bottom surface from the source and drain ends at a portion formed with a channel and a straight line in contact with the groove corner has a relationship with the doping concentration as shown in FIG. 1B. The threshold voltage is not reduced even when the channel length is decreased by adjusting the groove shape and the doping concentration.

Grooved gate transistor having source and drain diffused layers with

The instant invention is drawn to a method for manufacturing 2',3'-dideoxy-2',3'-didehydronucleoside compounds by reacting a ribonucleoside derivative wherein the hydroxyl in the 5'-position of the furanose ring of said ribonucleoside is protected by a silyl protective group, with an acid anhydride such as acetic anhydride in the presence of a catalyst such as hydrous zirconium oxide, and then subjecting the reaction product to decarboxylation. The decarboxylation can be effected by heating the reaction product. This method can be performed in a single step, and requires no reagents which are expensive or chemicals which should be handled with care. Hence, it serves to manufacture 2',3'-dideoxy-2',3'-didehydronucleosides easily at low cost.

Method of manufacturing 2',3'-dideoxy-2',3'-didehydronucleosides

This invention provides a process for producing 3'-deoxy-'-fluorothymidine, which comprises allowing 3'-deoxy-'-fluoro-5'-mesylthymidine (the stating material) to react with an acetylating agent, selected from the group consisting of alkali metal salts of acetic acid, amine salts of acetic acid and ammonium acetate, in an aprotic, ploar solvent to form the 5'-acetyl derivative, and ammonium acetate, in an aprotic, polar solvent to form the 5'-acetyl derivative, and eliminating the 5'-acetyl group from this intermediate, thereby giving the objective 3'-deoxy-'-fluorothymidine. According to the process of this invention described above, the 5'-mesyl derivative can be efficiently acetylated, and 3'-deoxy-'-fluorotymidine can be obtained in a high yield.

Process for producing 3'-deoxy-3-'-fluorothymidine

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Tokyo, Japan

Junko Tanaka