Agritope, Inc.

Judit Monis

An approximately 37 kilodalton (kd) protein associated with grapevine leafroll disease infected plants is disclosed. The 37 kd protein is the coat protein for a grapevine leafroll-associated virus designated GLRaV-8. The grapevine virus-encoded 37 kd polypeptide is immunologically distinct from the approximately 36 kd proteins associated with GLRaV-4 or GLRaV-5 or the approximately 38 kd protein associated with GLRaV-1. The invention further provides a substantially pure antibody directed against the 37 kd virus-associated protein, a stable cell line capable of producing such a monoclonal antibody, and a method for assaying for a virus infection in Vitis species. The method involves detecting the presence of a 37 kd polypeptide encoded by an RNA-containing plant virus using an antibody that does not react with a viral encoded polypeptide of ca. 38 kd.

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Beaverton, OR, United States of America

Judit Monis