Chiron Corporation

Joyce A Running

Michael S Urdea

Janice A Kolberg

Brian David Warner

Thomas Horn

Chu-an Chang

Patrick J Sheridan

Jennifer M Clyne

Ray Sanchez-Pescador

Bruce D Irvine

Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.

Nucleic acid probes immobilized on polystyrene surfaces

Novel DNA probe sequences for detection of HBV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.

HBV capture and amplifiers probes for use in solution phase sandwich

Process for immobilizing nucleic acid probes on polystyrene surfaces

Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.

Nucleic acid hybridization assay for hepatitis B virus DNA

Bifunctional blocked phosphoramidites useful in making nucleic acid

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Concord, CA, United States of America

Joyce A Running