Abbvie Inc.

Other

Abbott Laboratories Corporation

Joseph G Matuck

James Francis McIntire

John C Fann

David F Bruton

Itzcoatl A Pla

Thomas Seewoester

Christof Schulz

Nichole A Roy

Yu-Hsiang David Chang

David Chang Yu-Hsiang

Don Hemmavanh

Alane E Wentz

The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.

Modified serum-free cell culture medium

Protein glycosylation greatly influences the structure, function, and pharmacokinetics of recombinant proteins. Here, growth media supplemented with metal ions is shown to modulate the protein glycosylation profile of recombinant proteins expressed in a variety of eukaryotic cell lines. In particular, millimolar amounts of ferric salts (e.g. ferric nitrate, ferric citrate, etc.) significantly increased the percentage of galactosylated N-glycan species. The effect on protein glycosylation was concentration-dependent manner up to 1.0 mM across multiple cell lines without adverse effects on cell viability. The use of iron supplements in cell culture media provides an efficient and effective approach towards the specific re-targeting of N-glycan glycoform profiles of recombinantly expressed proteins.

Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins

Methods of producing adalimumab

Fed-batch methods for producing adalimumab

Fed-batch method of making anti-TNF-alpha antibody

Fed batch method of making anti-TNF-alpha antibodies

Fed-batch method of making human anti-TNF-alpha antibody

Fed-batch cell culture methods using non-animal-based hydrolysates

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Worcester, MA, United States of America

Joseph G Matuck