Isis Innovation Limited

Jonathan Mark Gleadle

Christopher Joseph Schofield

Peter John Ratcliffe

Luke McNeill

Christopher William Pugh

A method of identifying an agent which modulates hydroxylation of hypoxia inducible factor (HIF), comprises contacting a HIF asparagine hydroxylase and a test substance in the presence of a substrate of the hydroxylase under conditions in which asparagine in the substrate is hydroxylated in the absence of the test substance; and determining hydroxylation of the substrate. Preferably the substrate is a HIF polypeptide comprising HIF-1&#x3b1;, a fragment thereof comprising Asn 803 of HIF-1&#x3b1; or a peptide analogue of HIF-1&#x3b1; or fragment thereof comprising an asparagine equivalent to Asn 803 of HIF-1&#x3b1; and wherein hydroxylation of Asn 803 or of a said equivalent asparagine is determined.

Growing community of inventors

Oxford, United Kingdom

Jonathan Mark Gleadle