Stemcell Technologies Canada Inc.

Sqz Biotechnologies Company

Jonathan B Gilbert

Howard Bernstein

Armon R Sharei

Scott Loughhead

Finola Moore

Bu Wang

Tia Ditommaso

The present invention provides methods for inducing tolerance and/or suppressing an immune response to an antigen by passing a cell suspension containing an anucleate cell through a constriction, wherein the constriction deforms the cell thereby causing a perturbation of the cell such that an antigen and/or tolerogenic factor enters the cell. In some embodiments, the anucleate cell is delivered to an individual and the antigen is delivered to and processed in a tolerogenic environment to induce tolerance and/or suppress an immune response to the antigen.

Intracellular delivery of biomolecules to induce tolerance

The present disclosure pertains to methods for delivering a compound into a cell comprising a cell wall, including passing a cell suspension through a constriction, wherein said constriction deforms the cell comprising a cell wall, thereby causing a perturbation of the cell such that the compound enters the cell, wherein said cell suspension is contacted with the compound.

Intracellular delivery of biomolecules to cells comprising a cell wall

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Somerville, MA, United States of America

Jonathan B Gilbert