Promega Corporation

John W Schultz

Dieter Klaubert

Poncho Meisenheimer

James J Cali

James Unch

Martin Ken Lewis

William Daily

Frank Fan

Kristopher Zimmerman

Tonny Johnson

Michael P Valley

Michael Scurria

Laurie Engel

David L Leland

Laura L Bozek

Judith N Stevens

Braeden Butler

Erika Hawkins

Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

Cell lysis composition, methods of use, apparatus and kit

The present invention provides novel dyes for staining protein, particularly those in elecrophoretic gels. The dyes of the invention are derivatives of Coomassie.RTM. dyes. The invention also provides quantitative and qualitative methods for detecting proteins which comprise staining the proteins with a dye of the invention.

Protein staining compositions and methods

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Verona, WI, United States of America

John W Schultz