The Ohio State University

Other

John W Christman

Dehua Pei

Manjula Karpurapu

Patrick G Dougherty

Ziqing Qian

Described are peptides and peptide conjugates comprising CN binding motifs (CNBM) which inhibit the CN-NFAT interaction. In some embodiments, the peptides comprise: (i) CNBM; (ii) a hydrophobic, non-peptidic moiety (RH) which interacts with the hydrophobic pocket on a CN protein; (iii) a sequence -AAU1-AAU2-AAU3-AAU4-AAU5-AAU6-, wherein each of AAU2, AAU3, AAU4, AAU5, and AAU6, is, independently, optional, and each of AAU1, AAU2, AAU3, AAU4, AAU5, and AAU6 when present is independently an amino acid as defined herein; or (iv) combinations thereof. In some embodiments, RH is conjugated to the N- or C-terminus of the CNBM. In some embodiments, the sequence -AAU1-AAU2-AAU3-AAU4-AAU5-AAU6- is conjugated to the N- or C terminus of the CNBM. In some embodiments, the peptides comprise: CNBM and RH. In some embodiments. In some embodiments, the peptides comprise: CNBM and AAU1-AAU2-AAU3-AAU4-AAU5-AAU6-. In some embodiments, the peptides of the disclosure CNBM and RH.

Peptidyl inhibitors of calcineurin-NFAT interaction

Improved calcineurin inhibitors and methods of using these improved calcineurin inhibitors to inhibit calcineurin-NFAT signaling in cells and in subjects are disclosed.

Peptidyl calcineurin inhibitors

A sifting litter-box utilizing two identical litter trays(8a,8b), each with sieve openings(26a,26b) integrated into one sidewall(18a,18b). The trays are nested so that their respective integrated sieve openings(26a,26b) are on diametrically opposed sides of the nested pair of trays, thereby the backwall(22b) of the lower tray(8b) closes off the sieve openings(26a) of the upper tray(8a) when nested one atop the other. The litter(34) occupying only the top tray(8a) is, after use, sifted into the bottom tray(8b) by lifting the top tray(8a) and tilting its contents toward its sieve-wall(18a) allowing the reusable litter to pass through its sieve openings(26a) and form a static pile of sifted reusable litter(34) at the backwall(22b) of the bottom tray(8b). Feces and urine clumps(38) are retained in the top tray(8a) which in then emptied. The emptied top tray(8a) is sanitized and is nested as the bottom tray(8b), with the former bottom tray(8b) with the reusable litter pile becoming the top tray(8a) and once again the sieve-wall(18a) of the upper tray(8a) is closed off by the backwall(22b) of the lower tray(8b). The nested tray array is then shaken to level the sifted litter pile readying the sifting litter-box for reuse.

Growing community of inventors

Columbus, OH, United States of America

John W Christman