Stratos Genomics, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

John Tabone

Mark Stamatios Kokoris

Melud Nabavi

Aaron Jacobs

Samantha Vellucci

Drew Goodman

Jessica Vellucci

Dylan O'Connell

Kendall Berg

Jagadeeswaran Chandrasekar

Lacey Merrill

Dylan O'connell

The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

Enhancement of nucleic acid polymerization by aromatic compounds

Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed, including compounds having the following structure: wherein R, R, Rand Rare as defined herein. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.

Phosphoroamidate esters, and use and synthesis thereof

Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed, including compounds having the following structure: wherein R, R, Rand Rare as defined herein. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules

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Kirkland, WA, United States of America

John Tabone