Other

Baylor College of Medicine

Pharmacia Biotech Ab

Houston Advanced Research Center

John Shumaker

Daniel J Ehrlich

Mark Alexander Hollis

Michael E Hogan

Kenneth Loren Beattie

Mitchell D Eggers

C Thomas Caskey

Andres Metspalu

A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end therof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.

Parallel primer extension approach to nucleic acid sequence analysis

A method and apparatus for identifying molecular structures within a sample substance using an array having a plurality of test sites upon which the sample substance is applied. Each test site includes a probe formed therein to bond with an associated target molecular structure. An electrical signal is applied to the test site and the electrical properties of the test sites are detected to determine which probes have bonded to an associated target molecular structure.

Multi-site detection apparatus

Multi site molecule detection method

Growing community of inventors

Houston, TX, United States of America

John Shumaker