Wisconsin Alumni Research Foundation

John L Markley

Fariba Masoumeh Assadi-Porter

Bengt G Hellekant

Marco Tonelli

William M Westler

Qiu Cui

Ian A Lewis

Gabriel Cornilescu

Zheyuan Jin

A method of determining a concentration level of a compound in a mixture is provided. A plurality of compounds are identified in the mixture based on intensity peaks identified from NMR data generated from the mixture. A compound is selected from the identified plurality of compounds for a concentration determination. A concentration equation associated with the selected compound is determined. A first peak associated with the selected compound is selected from the NMR data. The selected first peak does not overlap with a peak associated with another compound of the identified plurality of compounds. An intensity value associated with the selected first peak is determined. A concentration of the selected compound in the mixture is calculated using the determined concentration equation and the determined intensity value.

Method and system for determining compound concentrations in a mixture using regression of NMR intensity peak values

Disclosed herein are sweet proteins that are variants of Brazzein, and nucleotide sequences capable of expressing them. Through a replacement of a particular amino acid in the naturally occurring Brazzein sequence with Lys or Asn, the taste profile and sweetness strength are improved.

Protein sweetener

Disclosed herein are sweet proteins that are variants of Brazzein, and nucleotide sequences capable of expressing them. Through a replacement of an amino acid in the naturally occurring Brazzein sequence with Arg or Ala (or the addition of Arg or Ala), the taste profile and sweetness strength can be changed.

Growing community of inventors

Madison, WI, United States of America

John L Markley