Genencor International, Inc.

Other

John J Lauff

Walter Weyler

Dan J Wendt

Timothy C Dodge

James Breitfeller

D Bernie Steele

Louise Coogan

There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.

Microbial production of indigo

A novel microorganism has been isolated and identified as Agrobacterium sp., a gram-negative aerobe. The Agrobacterium sp. microorganisms has been found to efficiently degrade ferric chelates of aminopolycarboxylic acids, such as ethylenediaminetetraacetic acid and related compounds, found in aqueous waste solutions. A biologically pure culture of Agrobacterium sp. and a process for using the culture are disclosed.

Pure culture of Agrobacterium sp. which degrades ferric chelates

Degradation of ferric chelates by a pure culture of agrobacterium sp.

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Rochester, NY, United States of America

John J Lauff