Xoma Corporation

International Genetic Engineering

Jar-how Lee

Lindley C. Blair

Raju K. Koduri

Joachim L. Weickmann

Joachim L Weickmann

Jar-How Lee

Lindley Blair

Pradip Ghosh-Dastidar

Raju K Koduri

Lindley C Blair

Genes are disclosed which are capable of directing the synthesis in a selected host microorganism of two thaumatin I analogues both of which have the amino acid sequence of natural thaumatin I including an aspartate amino acid residue in the 113th position from the amino terminal end of the polypeptide and one of which additionally has a lysine amino acid residue substituted for asparagine in the 46th position from the amino terminal end.

DNA encoding (ASP 113) and (LYS 46, ASP 113) thaumatin I

Genes are disclosed which are capable of directing the synthesis in a selected host microorganism of thaumatin I polypeptides having improved taste characteristics characterized by having the amino acid sequence of natural thaumatin I including a lysine amino acid residue substituted for asparagine in the 46th position from the amino terminal end, an aspartate amino acid residue substituted for asparagine in the 113th position from the amino terminal end and an aspartate amino acid residue substituted for lysine at either the 97th or 137th residue from the amino terminal end.

DNA encoding (Lys.sup.46, Asp.sup.97, Asp.sup.113) and (Lys.sup.46, Asp.sup

Growing community of inventors

Los Angeles, CA, United States of America

Joachim L Weickmann