Hong Guan Ltd.

Beihao Stem Cell and Regenerative Medicine Research Institute Co., Ltd.

Jingyang Guan

Xu Zhang

Yang Zhao

Hongkui Deng

Honggang Li

Chun Yen Liu

Yao Fu

Ting Zhao

Pingping Hou

Junqing Ye

Yanqin Li

Chun Liu

Provided are chemical inducers of pluripotency (CIP) which include glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, cyclic AMP agonists and S-adenosylhomocysteine hydrolase (SAH) inhibitors or histone acetylators. A method of inducing pluripotency in a partially or completely differentiated cell by using such chemical inducers of pluripotency is also provided. The method includes: (i) contacting a cell with the CIPs for a sufficient period of time to result in reprograming the cell into a pluripotent stem cell having ESC-like characteristics (CiPSC). Isolated chemically induced pluripotent stem cells (CiPSCs) and their progeny, produced by inducing differentiation of the CiPSCs, can be used in a number of applications, including but not limited to cell therapy and tissue engineering.

Methods for reprograming non-pluripotent cells into pluripotent stem cells

The invention provides a composition and a method for inducing pluripotency in non-pluripotent eukaryotic cells. The composition comprises chemical inducers of pluripotency (CIPs) including glycogen synthase kinase (GSK) inhibitors, TGFp receptor inhibitors, cyclic AMP agonists, S-adenosylhomocysteine hydrolase (SAH) inhibitors, and optionally an agent that promotes histone acetylation. The method comprises contacting a cell with the CIPs for a sufficient period of time to reprogram the cell into a pluripotent stem cell.

Compositions and methods for reprograming non-pluripotent cells into pluripotent stem cells

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Beijing, China

Jingyang Guan