Nanjing Tech University

Nanjing University of Science and Technology

Nanjing Hanxin Pharmaceutical Technology Co., Ltd.

Zhenrun Environmental Science and Technology Co., Ltd.

Chinese Academy of Forestry

Amphastar Nanjing Pharmaceuticals Inc.

Nanjing Hanxin Pharmaceutical Inc

Jing Wang

Haoning Zhang

Song Chen

Yan Gu

Chuangen Tang

Xiaodong Liu

Yuxiang Chen

Jinyou Shen

Zhendong Zhao

Liangwu Bi

Xiaoqiang Chen

Yanju Lu

Shangshu Pan

Dongmei Li

Xiaorui Liu

Bo Jin

Yanyan Wang

Xiaoyang Fan

Cheng Hou

Xinbai Jiang

Junfeng He

Hebing Zhang

Hong Wang

Teng Zhang

Tingwen Wei

The present invention relates to the technical field of wastewater treatment, and discloses a bioaugmentation treatment process for lithium battery producing wastewater. The method comprises the following steps: 1) introducing wastewater into a hydrolytic acidification tank, and addingsp. NJUST50 and activated sludge to the hydrolytic acidification tank for hydrolytic acidification treatment; 2) introducing the effluent into an anoxic tank, and addingsp. NJUST50 and anaerobic activated sludge for anoxic treatment; 3) introducing the effluent into an aerobic tank, and addingsp. NJUST50 and aerobic activated sludge for aerobic treatment; 4) introducing the effluent into an anoxic filter tank, and addingsp. NJUST50 and anaerobic activated sludge to the filter tank for treatment; and 5) introducing the effluent into a biological aerated filter tank, and adding a sludge mixture ofsp. NJUST50 with aerobic activated sludge to the filter tank for treatment.

Bioaugmentation treatment process for lithium battery producing wastewater

The invention discloses a fusion protein and its preparation method for intermediate polypeptide of Semaglutide. The invention belongs to the technical field of genetic engineering and polypeptide preparation. The fusion protein comprises a fusion peptide, a protease cleavage site and a target main molecular sequence. By optimizing the fusion peptide sequence, changing the isoelectric point and hydrophilicity of the protein, the highest expression of the fusion protein is effectively increased to 13.1 g/L. Meanwhile, the properties of fusion protein are also improved, which is conducive to the development of subsequent extraction, enzyme digestion and purification processes. The yield of intermediate polypeptide after enzyme digestion is 3.62 g/L. The production cost of Semaglutide intermediate polypeptide Arg34GLP-1(9-37) is reduced from the source, which is conducive to industrial scale-up and suitable for industrial production.

Fusion protein and its preparation method for intermediate polypeptide of Semaglutide

The present disclosure provides a method for photocleavage of an amide bond, the method has mild reaction conditions and can realize the cleavage of amide bonds by using light. The method comprises the following steps: subjecting 2,4-dinitrofluorobenzene to a reaction with an amino group of a substance represented by structural formula I with an α-amino acid at the end to produce a compound 1 represented by structural formula II; then under light irradiation, subjecting the compound 1 to a cleavage reaction of amide bond; Wherein, R1 is the side chain group of α-amino acid; R2 is: aryl, aliphatic hydrocarbon, —CH(R)—COOH or polypeptide.

Method for photocleavage of amide bonds

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Nanjing, China

Jing Wang