Beijing Minhai Biotechnology Co., Ltd.

Jiankai Liu

Lin Guo

Guoshun Li

Meirong Gu

Lei Chen

Yingzhi Xu

Haifeng Xiao

Gaimei Zhang

Jin Li

Junjie Liu

Shanshan Xu

Wenjin Wei

Linlin Song

Wei Jian

Lili Zhao

Xuechao Xie

The present disclosure provides an engineeredfungus that efficiently expresses CA10 (Coxsackievirus A10) virus-like particles and uses thereof. The engineered fungus includes a recombinant vector carrying the P1 and 3CD genes of the CA10 virus optimized according to preferred codons of. The present disclosure also provides a preparation method for CA10 virus-like particles and vaccines prepared therefrom.

Engineered Hansenula fungi efficiently expressing CA10 virus-like particles and uses thereof

The present invention provides a method for purifying an EV71 virus-like particle and a method for preparing a vaccine thereof. The virus-like particle is obtained by performing high density fermentation cultivation on recombinantly engineered bacteria; inducing expression of the EV71 virus-like particle protein expression using methanol; collecting the bacteria by centrifugation and performing high-pressure homogenization for disruption; performing precipitation on the supernatant with ammonium sulfate; and purifying by redissolution, ultrafiltration, ion exchange chromatography, molecular sieve chromatography, hydroxyapatite chromatography, etc.

Purification of recombinant EV71 virus-like particle and method for preparing vaccine thereof

The present invention provides EV71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a P1 protein gene and a 3CD protease gene of an EV71 virus with a PMV plasmid to construct a PMV-P1-3CD recombinant expression plasmid; then transforming aAU-0501 expression strain with the PMV-P1-3CD recombinant expression plasmid to obtain an AU-PMV-P1-3CD recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the EV71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain EV71 virus-like particles.

EV71 virus-like particles and preparation method and application thereof

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Beijing, China

Jiankai Liu