Industrial Technology Research Institute

Jia-Hung Wang

Kelly Teng

Yu-Ju Lin

Hsin-Ju Hsieh

Meng-Yin Tsai

Ting-Ting Hung

Liang-Jung Chien

Shu-Hsien Tsai

Described herein are genetically-modified microorganisms for producing long-chain dicarboxylic acids and methods of using the microorganisms. The microorganisms contain a first nucleic acid encoding anlauroyl ACP-thioesterase (BTE) operably linked to a promoter or a second nucleic acid encoding alauroyl ACP-thioesterase (FatB3) operably linked to a promoter.

Genetically modified microorganism for producing long-chain dicarboxylic acid and method of using thereof

The invention provides a cell for preparing competent cells, wherein the cell is capable of spontaneously accumulating self-producing trehalose therein and the cell is used for the preparation of competent cells. The invention also provides a method for preparing competent cells, including: culturing the cell for preparing the competent cell mentioned previously to obtain a cell suspension; placing the cell suspension into an ice bath; centrifuging the cell suspension to obtain a cell precipitate; mixing a transform reagent with the cell precipitate; and obtaining competent cells suspension.

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Method for producing itaconic acid in yeast cells using glycerol as the substrate. The yeast cells express cis-aconitic acid decarboxylase and optionally, citrate synthase and/or aconitase at high levels.

Producing Itaconic acid in yeast using glycerol as the substrate

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Taichung County, Taiwan

Jia-Hung Wang