Molecular Devices Corporation

Mds Analytical Technologies (us) Inc.

Jesse J Twu

Zhenjun Diwu

Luke D Lavis

Yen-Wen Chen

Guoliang Yi

Kelly J Cassutt

Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2',7&#x2032;-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

Methods of using fluorescent pH indicators for intracellular assays

Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2&prime;,7&prime;-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

Fluorescent pH indicators for intracellular assays

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Cupertino, CA, United States of America

Jesse J Twu