Sri International

Jerome B Lazar

Paul H Johnson

Richard C Winant

David Gray Streeter

Nahid S Waleh

Indira Sohel

Ping Sze

The present invention provides a process for the recovery of heterologous proteins from CTAP-III fusion proteins comprising expressing a fusion protein having a first amino acid sequence, a second amino acid sequence, and a selectable site which may be cleaved to provide first and second polypeptide fragments, respectively, wherein the first amino acid fragment is homologous to CTAP-III, and the first and second fragments have different pI values; cleaving the fusion protein to provide the first and second fragments; and separating the first and second fragments by ion exchange chromatography.

Purification of proteins employing CTAP-III fusions

Human laminin B.sub.1 peptides have been produced. The invention provides novel peptides which contain substantial amino acid sequence similarity to amino acids 897 to 936 in the human sequence. These peptides have been found to have enhanced antimetastatic activity.

Antimetastatic peptides with laminin activity

A method for obtaining heterologous peptides from fusion proteins wherein at least one of the fusion components is connective tissue-activating peptide-III is provided. Hirudin, a laminin B.sub.1 peptide and platelet factor 4 are polypeptides expressed using this method. DNA sequences encoding the fusion protein, vectors containing these sequences and transformed prokaryotic hosts useful in practicing the method of the present invention are also provided.

Method and vectors for stabilizing hirudin and human laminin B.sub.1

Compounds and compositions comprising amino acid substituted and chemically modified analogs of hirudin are provided, together with methods for their production and use as antithrombotic and anticoagulant agents.

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Sunnyvale, CA, United States of America

Jerome B Lazar