Basf Plant Science Gmbh

Jelena Kirsch

Jörg Bauer

Petra Cirpus

Ernst Heinz

Amine Abbadi

Xiao Qiu

Thorsten Zank

Patricia Vrinten

Frederic Domergue

Petra Sperling

Astrid Meyer

Joerg Bauer

Jorg Bauer

The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with &#x394;-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a &#x394;-6 desaturase, a &#x394;-5 desaturase, &#x394;-4 desaturase and/or &#x394;-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived fromor. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated &#x3c9;-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of &#x394;-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of &#x3c9;-3 fatty acids, oils or lipids with &#x3c9;-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with &#x3c9;-3 desaturases, for example a &#x3c9;-3 desaturase from fungi of the family Pythiaceae such as the genus, for example, the genus and species, or a &#x3c9;-3 desaturase from algae such as the family Prasinophyceae, for example, the genusand, particularly, the genus and speciesor diatomace&#xe6; such as the genusand, particularly, the genus and species. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

Method for the production of multiple-unsaturated fatty acids in transgenic organisms

The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, the nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing the nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.

Method for producing polyunsaturated long-chain fatty acids in transgenic organisms

Growing community of inventors

Hamburg, Germany

Jelena Kirsch