Cpc International, Inc.

Jeffrey R Frankiewicz

Robert E Heady

Jay A Smith

Christopher J Lemme

A novel asporogenic strain of Clostridium acetobutylicum is produced by growing a spore-forming strain in a continuous culturing reactor. Culturing is conducted at a dilution rate which prevents accumulation of butanol and acetone in the medium. Culturing at this dilution rate is continued until the asporogenic strain is obtained.

Strain of Clostridium acetobutylicum and process for its preparation

An improved process for the continuous production of butanol by fermentation of carbohydrates with C. acetobutylicum is disclosed. This process combines continuous inoculum production at a high dilution rate and cycling the fermentation broth through a material which adsorbs butanol whereby a vigorous cell population is maintained in the fermentation reactor for extended periods of time.

Production of butanol by a continuous fermentation process

Glucoamylase is prepared by fermenting a microorganism capable of producing glucoamylase in a nutrient medium containing at least about 16% to about 25% of ground corn which has been liquefied by the action of heat, water and an alpha-amylase enzyme preparation. The alpha-amylase enzyme preparation is preferably derived from a microorganism of Bacillus licheniformis.

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Lombard, IL, United States of America

Jeffrey R Frankiewicz