Massachusetts Institute of Technology

Harvard College

The General Hospital Corporation

The Brigham and Women's Hospital, Inc.

Children's Medical Center Corporation

Jeffrey D Macklis

George M Church

Kwang Ho Lee

Haruhiko Harry Asada

Roger Dale Kamm

Seok Chung

Waleed Ahmed Farahat

Jeffrey Matthew Nivala

Ioannis K Zervantonakis

Levi B Wood

Seth Lawler Shipman

Chandrasekhar Kothapalli

Suzanne Tharin

Johanna Varner

Young Kum Park

Le Thanh Tu Nguyen

Choong Kim

This invention provides methods of altering a cell including providing the cell with a nucleic acid sequence encoding a Cas1 protein and/or a Cas2 protein of a CRISPR adaptation system, providing the cell with a CRISPR array nucleic acid sequence including a leader sequence and at least one repeat sequence, wherein the cell expresses the Cas1 protein and/or the Cas2 protein and wherein the CRISPR array nucleic acid sequence is within genomic DNA of the cell or on a plasmid. Also provided are methods and systems for nucleic acid storage and in vivo molecular recordings of events into a cell.

Methods and systems of molecular recording by CRISPR-Cas system

Provided herein are microfluidic devices that can be used as a 3D bioassay, e.g., for drug screening, personalized medicine, tissue engineering, wound healing, and other applications. The device has a series of channels {e.g., small fluid channels) in a small polymer block wherein one or more of the channels can be filled with a biologically relevant gel, such as collagen, which is held in place by posts. As shown herein, when the device is plated with cells such as endothelial cells, new blood vessels grow in the gel, which is thick enough for the cells to grow in three dimensions. Other channels, e.g., fluid channels, allow drugs or biological material to be exposed to the 3D cell growth. Cells, such as endothelial cells, can be cultured and observed as they grow on the surface of a 3D gel scaffold, where e.g., rates of angiogenesis can be measured, as well as intervascularization and extravascularization of cancerous cells.

Device for high throughput investigations of multi-cellular interactions

Growing community of inventors

Brookline, MA, United States of America

Jeffrey D Macklis